技術(shù)文章
色譜柱1:4.6 x100 mm, HALO Peptide ES-C18, 2.7 µm 160A孔徑;貨號(hào):92124-602
色譜柱2:4.6 x 100 mm, HALO Protein ES-C18, 3.4 µm 400A孔徑;貨號(hào):93414-602
流動(dòng)相:70/30 A/B
A =0.1% TFA水溶液;B=0.1% TFA乙腈溶液
流速:1.5 mL/min
柱壓:見圖譜
柱溫:60°C
檢測(cè)器:UV215nm, VWD
進(jìn)樣體積:5.0μL
樣品溶劑:流動(dòng)相A
響應(yīng)時(shí)間:0.02 sec.
流通池:5 μL semi-micro
LC系統(tǒng):Agilent 1100
色譜柱1:4.6 x100 mm, HALO Peptide ES-C18, 2.7 µm 160A孔徑;貨號(hào):92124-602
色譜柱2:4.6 x 100 mm, HALO Protein ES-C18, 3.4 µm 400A孔徑;貨號(hào):93414-602
流動(dòng)相:70/30 A/B
A =0.1% TFA水溶液;B=0.1% TFA乙腈溶液
流速:1.5 mL/min
柱壓:見圖譜
柱溫:60°C
檢測(cè)器:UV215nm, VWD
進(jìn)樣體積:5.0μL
樣品溶劑:流動(dòng)相A
響應(yīng)時(shí)間:0.02 sec.
流通池:5 μL semi-micro
LC系統(tǒng):Agilent 1100